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Mycoplasma is a common contaminant in mammalian cell culture processes, mycoplasma contaminant can cause changes in host cell DNA, RNA and protein expression, such as interruption of DNA and RNA synthesis and changes in membrane antigens, thus affecting cell growth and proliferation. ResiQuant® Mycoplasma DNA Extraction and Purification Kit (Magnetic Bead Method) effectively reduces the interference of cells and sample matrix through pretreatment and specific binding of magnetic beads, and can extract and purify trace amounts of mycoplasma DNA in biological samples. The extraction step is simple and only takes 2~3 hours. Used in conjunction with the ResiQuant® Mycoplasma DNA Detection Kit (Fluorescent Probe qPCR Method)（CAT. CRB00-1011/CRB00-1012）, it can achieve the purpose of rapid detection of mycoplasma.
It is recommended to conduct a suitability study when using this kit for the first time to confirm whether the test sample matrix interference with the extraction and detection of DNA. Direct extraction or centrifugal pretreatment auxiliary methods are selected based on the results of the suitability study. According to the Chinese Pharmacopoeia (2022 edition), the recommended sampling volume for the mycoplasma examination and culture method is 0.5~1 mL, this kit can directly process samples of 1mL and below, and large-volume samples can be extracted through centrifugal pretreatment.
Product Features Universality
It can process a variety of matrix samples, including cells, cell culture supernatant, serum, serum-free medium, cell cryopreservation solution, etc; Compatibility
Compatible with ResiQuant® Mycoplasma assay kit; Simplicity
No need for cumbersome operating steps, simplify the experimental process; Short Time
The extraction step is simple and only takes 2~3 hours;
Product Advantages Complete kit traceability system
The components are independently prepared and developed, the raw materials are independently controllable, and the kit traceability system is complete; Easy to use
The extraction step only takes 2~3h, and the operation steps are simple; One-stop service
Paired with the ResiQuant® Mycoplasma Detection Kit (Quantitative qPCR Method), it completely solves the problem of cellular mycoplasma detection; Supply Chain Security
The batch output is large, the delivery time is short, and the supply is stable;
Product Properties
Product Standards
Separation Technology
Magnetic beads
Sample type
Cellular or non-cellular samples
Final product type
Genomic DNA purification
Target organic species
Mycoplasma
Testing time
2-3 hours
Sampling volume
0.5-1mL
Cell mass
1×106~1×107
Storage conditions
Box 1 components are stored at room temperature, Box 2 components are stored at -40℃ to 18℃.
Validity period
Can be stored for 12 months under specified storage conditions.
Shipping conditions
Box 1 is transported at normal temperature and Box 2 is transported on dry ice.
The kit can be used for micro-extraction of mycoplasma nucleic acid from biological samples. It is suitable for samples such as cell culture supernatant, bovine serum, serum-free medium and cell cryopreservation fluid. It is also suitable for samples such as cell banks and therapeutic cells. Directly process cell samples of 1×106~1×107.
